PIPseq™ Unique Dual Index (UDI) – 96 Kit
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Included in This Kit
The PIPseq™ UDI-96 Kit enables multiplex sample preparation of PIPseq Single Cell 3ʹ gene expression libraries for next-generation sequencing on the Illumina platform. During the sample index PCR step of PIPseq 3ʹ gene expression library preparation, sample barcodes are incorporated by use of the UDI-96 Kit, thereby enabling multiplexing.
Each kit provides 96 pre-mixed unique pairs of i5 and i7 index primers (10-base index) packaged in a single-use 96-well breakable plate with a pierceable foil seal. This kit is only intended and validated for use with the PIPseq 3’ Single Cell RNA Kits (T2, T20, and T100).
Key Benefits
- Enables multiplex sample preparation for Illumina NGS platform for up to 96 samples
- Pre-mixed index primers allows for quick and error-free setup
- Break apart strip design enables efficient usage based on number of samples
Downloads
Users may download this “import sample template” for upload into Illumina BaseSpace Run Setup which contains the PIPseq UDI-96 Kit sequences.
Note that Illumina requires specific configurations of adapter sequences in the sample sheets for different sequencing platforms. Users may download the following index sequence sheet to aid in creation of an appropriate sample sheet, however, users must take caution as this sheet is not formatted for direct upload to an Illumina sequencing instrument. Please refer to Indexed Sequencing on Illumina Systems (doc ID: 15057455) for more information on creation of valid sample sheets for specific Illumina sequencing platforms.
Related Resources
Select from the below resources to learn more about our protocols (user guides) or customer applications (posters).
Single Cell RNA Kit Application Areas
Single-cell RNA sequencing (scRNA-Seq) has enabled unprecedented insight into the biology of individual cells and tissue complexity across a broad range of discovery and disease areas. Below are some of the application areas where the the accessibility, precision, and sensitivity of our PIPseq technology can enable new research and discoveries
Cancer
Immunology
Neuroscience
Infectious Disease
PIPseq™ PIPseq Unique Dual Index (UDI) – 96 Kit
- 96 pre-mixed unique pairs of i5 and i7 index primers packaged in a single-use 96-well breakable plate
- Enables multiplex sample preparation of PIPseq libraries on Illumina NGS instruments
- Compatible with all PIPseq 3’ Single Cell RNA Kits (T2, T20 and T100)
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